| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methylocella silvestris | TGCGCTTCAGACTGTCTCC | ACAACCCCTCGACGATCAC | 59.71 | 59.41 | 186 | 169 |
100.00%
|
100.00%
|
| Primer 2 | Methylocella silvestris | GGGCTTTCCCGTTTTCGTC | ACAACCCCTCGACGATCAC | 59.42 | 59.41 | 164 | 158 |
100.00%
|
100.00%
|
| Primer 3 | Methylocella silvestris | TTTCGCGAGCAGGACCAT | TCAGGCGTTGAGGGTTTCC | 59.34 | 59.93 | 258 | 149 |
100.00%
|
100.00%
|
| Primer 4 | Methylocella silvestris | TCCGAAACGCAAGACCTCC | GGCTTCCTTGTTCTTCGGC | 60.01 | 59.12 | 178 | 143 |
100.00%
|
100.00%
|
| Primer 5 | Methylocella silvestris | TTTTCGCAGCGCTTGAGC | CGACCGAAAACGGCTTTCC | 59.75 | 59.79 | 171 | 143 |
100.00%
|
100.00%
|
| Primer 6 | Methylocella silvestris | TCCCGTCGTCGTGGATTTC | TGCCGTCCTTGAACAGCA | 59.79 | 59.49 | 191 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Methylocella silvestris | CCACGTCAACAATGCGGTG | GCGTTGCGATCACAACCTC | 60.08 | 59.87 | 170 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Methylocella silvestris | AACGCCTGATCTACGCCTC | AGCCATGCGTGCTGTCAT | 59.56 | 60.05 | 204 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Methylocella silvestris | TGCGATTCTCGGCAAGCA | TTGAGGCGAAGGTCGGTTG | 60.05 | 60.30 | 207 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Methylocella silvestris | TGCGATTCTCGGCAAGCA | TGATGTTGGGGAGGCTTGC | 60.05 | 60.30 | 239 | 138 |
100.00%
|
100.00%
|